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ctnt reafinitytm conjugated with fitc  (Miltenyi Biotec)


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    Miltenyi Biotec ctnt reafinitytm conjugated with fitc
    Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker <t>cTnT</t> and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.
    Ctnt Reafinitytm Conjugated With Fitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ctnt+reafinity+conjugated+fitc/Cardiac+Troponin+T+Antibody%2C+anti-human%2Fmouse%2Frat%2C+REAfinity/pmc13002374-59-16-22
    Average 96 stars, based on 112 article reviews
    ctnt reafinitytm conjugated with fitc - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "GJA5 and ATP1A1 perturbations recapitulate inflammation-related beat irregularities in iPSC-based atrial myocardium tissue model"

    Article Title: GJA5 and ATP1A1 perturbations recapitulate inflammation-related beat irregularities in iPSC-based atrial myocardium tissue model

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1719392

    Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.
    Figure Legend Snippet: Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.

    Techniques Used: Activation Assay, Injection, Single Cell, MANN-WHITNEY, Staining, Marker

    Related Articles

    Incubation:

    Article Title: Immune response caused by M1 macrophages elicits atrial fibrillation-like phenotypes in coculture model with isogenic hiPSC-derived cardiomyocytes.
    Article Snippet: Flow cytometry was performed using a Novocyte Flow Cytometer 200 (ACEA Biosciences), with all washes and dilutions performed using FACS buffer (Ncardia). .. Suspended, previously fixed aCM and vCM of both lines were co-stained with antibodies for cTnT Reafinity conjugated FITC (1:10, Miltenyi) and MLC2a Reafinity conjugated APC (1:10, Miltenyi) with an incubation of 15 min at RT. ..

    Article Title: Immune response caused by M1 macrophages elicits atrial fibrillation-like phenotypes in coculture model with isogenic hiPSC-derived cardiomyocytes
    Article Snippet: Flow cytometry was performed using a Novocyte Flow Cytometer 200 (ACEA Biosciences), with all washes and dilutions performed using FACS buffer (Ncardia). .. Suspended, previously fixed aCM and vCM of both lines were co-stained with antibodies for cTnT Reafinity conjugated FITC (1:10, Miltenyi) and MLC2a Reafinity conjugated APC (1:10, Miltenyi) with an incubation of 15 min at RT. ..

    Article Title: GJA5 and ATP1A1 perturbations recapitulate inflammation-related beat irregularities in iPSC-based atrial myocardium tissue model
    Article Snippet: Samples were incubated with secondary antibodies (IgG (H+L) Alexa Fluor ® 488 goat anti human (1:500, ThermoFisher Scientific), Goat anti-Mouse IgG2b, Alexa Fluor ® 647 conjugated (1:200, ThermoFisher Scientific)) for 1h at RT, before being washed and imaged. .. Tissues were incubated with primary antibodies (cTnT REAfinity conjugated FITC (1:100, Miltenyi Biotec)) and CX3CR1 rabbit anti-human (1H14L7) (1:250, Invitrogen) or Collagen I Polyclonal Antibody (1:100, ThermoFisher Scientific) overnight at 4 °C on a plate shaker. .. Samples were incubated with secondary antibodies (IgG (H+L) Alexa Fluor ® 488 goat anti human (1:200, ThermoFisher Scientific), IgG (H+L) Alexa Fluor ® 594 Donkey anti Rabbit (1:200, ThermoFisher Scientific), Donkey Anti-Rabbit IgG (H+L) antibody Alexa Fluor ® 488 (1:200, ThermoFisher Scientific) and DAPI (1:1000, Invitrogen) for 1h at RT, before being washed and imaged.



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    Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker <t>cTnT</t> and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.
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    Image Search Results


    Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.

    Journal: Frontiers in Immunology

    Article Title: GJA5 and ATP1A1 perturbations recapitulate inflammation-related beat irregularities in iPSC-based atrial myocardium tissue model

    doi: 10.3389/fimmu.2025.1719392

    Figure Lengend Snippet: Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.

    Article Snippet: Fixed aCM in suspension were co-stained with MLC2a REAfinityTM conjugated with APC (1:10, Miltenyi Biotec) and cTnT REAfinityTM conjugated with FITC (1:10, Miltenyi Biotec) antibodies and incubated for 15 min at RT.

    Techniques: Activation Assay, Injection, Single Cell, MANN-WHITNEY, Staining, Marker

    Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.

    Journal: Frontiers in Immunology

    Article Title: GJA5 and ATP1A1 perturbations recapitulate inflammation-related beat irregularities in iPSC-based atrial myocardium tissue model

    doi: 10.3389/fimmu.2025.1719392

    Figure Lengend Snippet: Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.

    Article Snippet: Tissues were incubated with primary antibodies (cTnT REAfinity conjugated FITC (1:100, Miltenyi Biotec)) and CX3CR1 rabbit anti-human (1H14L7) (1:250, Invitrogen) or Collagen I Polyclonal Antibody (1:100, ThermoFisher Scientific) overnight at 4 °C on a plate shaker.

    Techniques: Activation Assay, Injection, Single Cell, MANN-WHITNEY, Staining, Marker

    EC-EVs enhance cardiomyocyte coverage in vascularized cardiac tissues. (A) Representative immunostaining images of cardiac troponin T (cTnT) highlighted the morphology of cardiomyocytes in EC/DPSC/CM tissues alongside those supplemented with EC-EVs. Day 10, scale bars: 200 μ m. (B)‒(D) Quantification of (B) normalized area fraction, (C) normalized integrated density, and (D) weighted mean eccentricity of cTnT immunostaining. Statistical analysis was performed by student t-tests ( P < 0.05 considered significant). N = 3 for EC/DPSC/CM, N = 4 for EC/DPSC/CM + EC-EVs. Data presented as mean ± standard deviation (SD).

    Journal: Biofabrication

    Article Title: Endothelial extracellular vesicles enhance vascular self-assembly in engineered human cardiac tissues

    doi: 10.1088/1758-5090/ad76d9

    Figure Lengend Snippet: EC-EVs enhance cardiomyocyte coverage in vascularized cardiac tissues. (A) Representative immunostaining images of cardiac troponin T (cTnT) highlighted the morphology of cardiomyocytes in EC/DPSC/CM tissues alongside those supplemented with EC-EVs. Day 10, scale bars: 200 μ m. (B)‒(D) Quantification of (B) normalized area fraction, (C) normalized integrated density, and (D) weighted mean eccentricity of cTnT immunostaining. Statistical analysis was performed by student t-tests ( P < 0.05 considered significant). N = 3 for EC/DPSC/CM, N = 4 for EC/DPSC/CM + EC-EVs. Data presented as mean ± standard deviation (SD).

    Article Snippet: Flow cytometry results were analyzed in FlowJo TM v10.10 Software (BD life sciences) [ ]. cTnT expression in iPS-CM differentiation culture was assessed using a similar preparation and flow cytometry protocol, with the inclusion of isotype control (1:50, Miltenyi Biotech 130-118-354) and using FITC-conjugated cTnT antibody (1:50, Miltenyi Biotech 130-119-575).

    Techniques: Immunostaining, Standard Deviation